7th IMRMPT

Abstract

Abstract information

English title:	BIODEGRADABILITY OF CASSAVA STARCH-BASED BIOFILMS EXPOSED IN SIMULATED SOIL
Spanish title:	BIODEGRADABILIDAD DE BIOPEL�CULAS BASADAS EN ALMID�N DE YUCA (CASSAVA STARCH) EXPUESTAS EN SUELO SIMULADO
English abstract:	Biodegradability is a parameter that allows us to determine and measure the environmental behavior of chemical substances, to determine the properties of the products that are intended to be released into the natural environment. Studying the biodegradation of polymers based on renewable sources becomes important since these processes allow us to identify structural changes in the compound or imply its conversion into inorganic products of low molecular weight. In this aspect, biofilms based on starch were synthesized via a thermal method, using (1.0 g), GLY (1.0 g), PEG (1.0 g), and GLU (1.0 g) chemical compounds; starch biofilms were tested for biodegradability, using simulated soil prepared with 23% silty silt, 23% organic matter (cow excrement), 23% sand, and 31% distilled water, biofilm samples were exposed to the simulated soil and packed in sealed perforated bags. The bags were buried in the ground 10 cm deep. Biodegradation was monitored for 3 weeks, every 3 days mass variation was measured, buried samples were recovered, washed with distilled water, dried at room temperature, and weighed, after measuring the mass was analyzed by FTIR, and the samples were again buried. The experiments were conducted in triplicate. The biofilms from the third day began the loss of mass due to the decrease in moisture of the polymer, after 8-10 days the mass loss was 68%-75% concerning the initial mass, by the action of endoenzymes of the microorganisms present in the organic matter, in which break the bonds of the internal chain randomly, resulting in a rapid decrease in molecular mass, due to the sequential cleavage of the terminal segments, leading to immediate changes, forming smaller molecules such as monomers and oligomers. After 13 days, tiny samples were obtained, and the mass loss corresponded to 99% of the initial mass, finally, on days 15 and 16 were no traces of biopolymers. FTIR analyses showed the functional groups' intensity loss, due to biodegradation. Therefore, the biodegradability tests demonstrated that starch polymers are biodegradable in simulated soil, the biodegradation period was between 13-16 days, and organic matter plays a key role in the process thanks to the presence of microorganisms, which break bonds decreasing the molecular mass of polymers transforming them into monomers assimilable in the medium. Likewise, the authors acknowledge Ministerio de Ciencia, Tecnolog�a e Innovaci�n (Minciencias) for funding through project BPIN 2020000100027 with resources from the SGR (Sistema General de Regal�as, Royalties General System). Moreover, the Universidad de Sucre is acknowledged for the financial support provided through the Call for Strengthening Research Groups of the Universidad de Sucre 2018-2019; approved by resolution No.16 of 2020 of the Academic Council.
Spanish abstract:	La biodegradabilidad es un par�metro que permite determinar y medir el comportamiento ambiental de las sustancias qu�micas, con el fin de determinar las propiedades de los productos que se pretenden liberar al medio ambiente natural. Estudiar la biodegradaci�n de pol�meros basados en fuentes renovables resulta ser importante, ya que estos procesos permiten identificar cambios estructurales del compuesto, o implicar su conversi�n en productos inorg�nicos de bajo peso molecular. En este aspecto, se sintetizaron unas biopel�culas basadas en almid�n (1,0 g), GLY (1,0 g), PEG (1,0 g) y GLU (1,0 G), usando el m�todo t�rmico; a estas se le realizaban pruebas de biodegradabilidad, usando suelo simulado preparado con 23% de limo limoso, 23% de materia org�nica (esti�rcol de vaca), 23% de arena y 31% de agua destilada, las muestras de biopel�cula se expusieron al suelo simulado y se empacaron en unas bolsas sellables agujereadas. Las bolsas se enterraron en el suelo a 10 cm de profundidad. La biodegradaci�n se control� durante 3 semanas, cada 3 d�as se med�a la variaci�n de masa, las muestras enterradas se recuperaron, se lavaron con agua destilada, se secaron a temperatura ambiente y se pesaron, despu�s de medir la masa se analizaba mediante FTIR, las muestras nuevamente fueron enterradas. Los experimentos se realizaron por triplicado. Las biopel�culas a partir del tercer d�a iniciaba la p�rdida de masa debido a la disminuci�n de humedad del pol�mero, al transcurrir los 8-10 d�as la masa perdida fue del 68%-75% en relaci�n a la masa inicial, por acci�n de endoenzimas de los microorganismos presentes en la materia org�nica, los cuales rompen los enlaces de la cadena interna al azar, lo que da como resultado una r�pida disminuci�n de la masa molecular, debido a la escisi�n secuencial de los segmentos terminales, conduciendo a cambios inmediatos, form�ndose mol�culas m�s peque�as como mon�meros y olig�meros. Al transcurrir los 13 d�as se obtuvieron muestras diminutas, la p�rdida de masa correspond�a al 99% de la masa inicial, por �ltimo, al d�a 15 y 16 no hab�a rastros de los biopol�meros; los an�lisis de FTIR mostraban la p�rdida de intensidad de los grupos funcionales, a causa de la biodegradaci�n. Por consiguiente, las pruebas de biodegradabilidad permitieron demostrar que los pol�meros de almid�n son biodegradables en suelo simulado, el periodo de biodegradaci�n fue entre 13-16 d�as, la materia org�nica juega un papel clave en el proceso gracias a la presencia de microorganismos, los cuales rompen enlaces disminuyendo la masa molecular de los pol�meros transform�ndolos en mon�meros asimilables en el medio. asimismo, los autores Agradecimiento a Ministerio de Ciencia, Tecnolog�a e Innovaci�n (Minciencias) por el financiamiento del proyecto identificado con BPIN 2020000100027 con recursos de la SGR - Sistema General de Regal�as y tambi�n a la Universidad de Sucre por el apoyo financiero brindado a trav�s de la Convocatoria de Fortalecimiento de Grupos de Investigaci�n de la Universidad de Sucre 2018-2019; aprobado por resoluci�n No.16 de 2020 del Consejo Acad�mico.
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Authors

Name	Institution	Email
Alvaro Arrieta Almario	Universidad de Sucre	alvaro.arrieta@unisucre.edu.co
Manuel Fernando Acosta Hum�nez	Universidad de C�rdoba	manuel.acosta@unisucrevirtual.edu.co
Jorge Ducuara	Universidad de Sucre	jaducuarah@gmail.com

Presentation

Room:	Principal Room
Date:	June 9, 2023
Hour:	11:20 - 11:40

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7^th IMRMPT
Seventh International Meeting for Researchers in Materials and Plasma Technology

Previous editions:

        1st IMRMPT 2011
        2nd IMRMPT 2013
        3rd IMRMPT 2015
        4th IMRMPT 2017
        5th IMRMPT 2019
        5+1 IMRMPT 2021

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